Point mutations and NNK site saturation libraries of PtGSTF8 were constructed by overlap extension PCR as previously reported 49 using primers T7 fw, T7 term and: PtGSTF8 (V12X): V12C13_rv and V12X_fw, PtGSTF8 (C13X): V12C13_rv and C13X_fw, PtGSTF8 (C13S): V12C13_rv and C13S_fw, PtGSTF8 (N108X): N108_rv, N108X_fw, PtGSTF8 (F112X): F112_rv, F112X_fw (Supplementary Table 4) using pANT35 as template
Rai A, Tripathi P, Dwivedi S, Dubey S, Shri M, Kumar S, Tripathi PK, Dave R, Kumar A, Singh R, Adhikari B, Bag M, Tripathi RD, Trivedi PK, Chakrabarty D, Tuli R (2011) Arsenic tolerances in rice ( Oryza sativa ) have a predominant role in transcriptional regulation of a set of genes including sulphur assimilation pathway and antioxidant system
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10.1186/s12906-019-2439-2 105 WuC
ERK is mainly implicated in cell proliferation and Rac1 in cell migration, while p38 MAPK and JNK are involved in differentiation and apoptosis