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Sequences of four successive pulses were performed

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The primary antibodies were as follows: B220 (Rat, 1:200, Biolegend #103202), CD11b (Rabbit, 1:200, Abcam #133357), CD3 (Rabbit, 1:200, Abcam #16669), CD4 (Rat, 1:200, ThermoFisher #14-9766-82), CD8 (Rat, 1:100, ThermoFisher, #14-0808-82), IBA1 (Rabbit, 1:200, VWR #100369-764), and MPO (Rabbit, 1:500, Abcam, #ab208670)

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