Approximately 105 cells of CO23, CO23MCFG and CO23RFLC strains were serially diluted and spotted onto YPD plates treated or not with 4 mM H2O2 in absence (1) or in presence of GSH precursors 0.02 M glutamate, 0.02 M cysteine, 0.02 M, glycine (2) or with 5 mM BSO (L-buthionine-sulfoximine) (3).
Alcohol depletes the bodys reserve of glutathione, especially in the liver
Thus, in this experiment, we used the structurally distinct HDM2 inhibitor MI-773, which like nutlin-3 potently suppressed ferroptosis (Fig 1C)
doi:10.1038/nchembio.212, PMID: 19718041 Sido B, Braunstein J, Breitkreutz R, Herfarth C, Meuer SC (2000) Thiol-mediated redox regulation of intestinal lamina propria T lymphocytes
BPC-157s impact here would likely be mediated through tissue repair, blood flow regulation, and inflammation management rather than immediate alterations to seminal protein secretion