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Besides, tobacco is rich in sugars (constituting 25%50% of dry weight) and proteins (26%29% of dry weight at the seedling stage and 12%15% of dry weight at the mature stage), as well as phytosterols such as -sitosterol, stigmasterol, and rape sterols

Functionally-important mutations in key genes involved in, e.g

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**Light tamping**: After each draw, lightly tamp the tobacco to maintain a constant burn.5

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