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79 Chronic hyperglycemia activates mTORC1 and reduces mTORC2 activity 157 in islet cells, which leads to the translocation of FOXO1 to nucleus and less nuclear PDX1, resulting in reduced insulin synthesis and secretion and impaired cell mass 80,158 (Fig

doi: 10.1166/jbn.2016.2234 96 NeequayeMStavnstrupSHarwoodWLawrensonTHundlebyPIrwinJet al

Clean handling, careful storage, and tidy reconstitution keep the process stable