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The BsmBI-digested, dephosphorylated vector was gel-purified and ligated with annealed, phosphorylated oligonucleotides

P.VremanH

Even though they can be commercially acquired as a cell line, being readily characterized and accessible, one disadvantage lies in the fact that they may not express all the surface molecules as primary cells do, due to the immortalization process, making them a less reliable model of the in vivo behavior [88]

Once again, the true answer to this question will depend on the individual, as the research has clearly validated that different delivery systems can all be effective