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Then, 1.5 mL of extraction buffer (80% methanol with 0.1% formic acid, v/v) was added, with vortexing for sample resuspension

doi:10.1007/s00726-010-0510-x Kranich O, Dringen R, Sandberg M, Hamprecht B (1998) Utilization of cysteine and cysteine precursors for the synthesis of glutathione in astroglial cultures: preference for cystine

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23, 24 Caspase-3, caspase-9, poly ADP ribose polymerase and anti-rabbit immunoglobulin G horseradish peroxidase antibodies were from Cell Signaling (Danvers, MA, USA)

But if you add only 1mL, that same 10 units now contains 0.5mg, double the dose