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doi: 10.1038/s41586-018-0023-4 381 WheelerM

Metabolite extraction was performed using the methanol/chloroform/water method, and quantification was conducted by Gas ChromatographyMass Spectrometry (GC-MS), as described previously 62

After washing 3 20min with 1ml 0,2% gelatin-TBS secondary antibody incubation occurred as described for the primary antibody and the coverslips were incubated for one hour at room temperature

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So let's actually look at what BPC-157 is, what it does, and whether the hype matches the reality