The PDB structures with the following accession codes 2WCI, 3L4N, 3D4M, 3D5J, 2M80, 2WUL, 2WOU, 1MEK, 1B4Q and 4FIW were used in this work
Following the appropriate incubation period, free thiol groups were blocked with 2 mM N -ethylmaleimide (NEM) for 15 min at 37 C
*Correspondence: Aixiang Huang, [email protected] These authors have contributed equally to this work Disclaimer All claims expressed in this article are solely those of the authors and do not necessarily represent those of their affiliated organizations, or those of the publisher, the editors and the reviewers
doi: 10.1007/s00216-020-02403-3 80 AyaO-SKentaHYoshikiSTakuyaTShihoT-SMinamiIet al
Companion Supplies & Routine Support GLOW Peptide Reconstitution Guide Choose Your Reconstitution Format Math is different for the pre-blend versus three separate vials