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After the cells reached ~ 80% confluence under normal culture conditions, the medium was changed to glucose-free DMEM (Gibco) and the dish was placed in an anaerobic chamber (5% CO2, 95% N2) to induce OGD-related cell injury

Critical Considerations: Investigational Use Only: These doses were used ONLY within specific clinical studies

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Regardless, amylin without question reduces food intake and in other studies, binge/hedonic eating behaviors

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