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Supernatants were collected and the protein concentrations were determined using a BCA assay kit (Pierce/Thermo Scientific, Rockford, IL) for Western blot analysis and high performance liquid chromatography (HPLC) assay

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ZDHHC11 mediates the S-palmitoylation of ATG2A In mammals, the S-palmitoylation of proteins is primarily catalyzed by S-acyltransferases that contain a zinc-finger DHHC motif (ZDHHC), which comprises 23 members (Linder and Deschenes, 2007)

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FDR-controlled metabolite annotation for high-resolution imaging mass spectrometry