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Cells were first incubated for 30 min at 37 C in HBSS supplemented with 20 mM HEPES, pH 7.4 and 50 M cycloheximide, then Palmostatin B in DMSO was added to a 50 M final concentration, and cells were immediately recorded at one image per 30 s for 45 min in the spinning disk confocal system described above

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We used the force field parameters for single-protonated phosphorylated Ser (S1P) and Thr (T1P) residues developed by Sticht and co-workers 59

In addition, mild acidosis (pH 6.6) has been shown to activate acid-sensing ion channel 1a (ASIC1a), inducing necroptosis in glioblastoma stem cells [95]

Journal of Neural Transmission, 108(5-6), 581-592