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A clear isosbestic point at 425 nm separates the two peaks further indicating two equilibrating molecular species (Figure 2B)

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Furthermore, when we resolved the affinity purified P complexes assembled in the absence or presence of ICP27 by glycerol gradient sedimentation, we observed that the P complexes assembled in the presence of ICP27 failed to reach higher density fractions and spread out into more gradient fractions (Supplementary Fig

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