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Description

Gene expression analysis Total RNA was extracted from samples using RNeasy Mini Kit (Qiagen, Hilden, Germany) and used for complementary DNA synthesis using the ReverTra Ace quantitative RT-PCR Kit (Toyobo, Osaka, Japan)

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10.1038/s41467-019-10881-y 83 HasanM.KannaM

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SF: Writing original draft, Writing review & editing

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Nutrients, 12 (4), 1181

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After reaction with the relevant reagents, the absorbance of BUN was read at 640 nm and the absorbance of serum creatinine was read at 546 nm using a SpectraMax M2e plate reader (Molecular Devices)

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