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After passing through the 70 m cell strainer (BD Falcon, Bedford, MA, United States), the cell suspension was centrifuged at 300 g for 5 min

Cell surface-bound IL-1alpha is an upstream regulator of the senescence-associated IL-6/IL-8 cytokine network

While the biological plausibility exists, the evidence does not meet the standard we would require to confirm anti-aging effects

Riancho J, Delgado-Alvarado M, Andreu MD, Paz-Fajardo L, Arozamena S, Gil-Bea FJ, et al

Dysregulated mitochondrial genes and networks with drug targets in postmortem brain of patients with posttraumatic stress disorder (PTSD) revealed by human mitochondria-focused cDNA microarrays