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The methods thus developed were applied to this archaeological case study, which was developed in a collaboration with members of the Nez Perce Tribe and Culture Department staff who requested residue analysis of archaeological pipes from their ancestral sites so that they may better document traditional use of tobacco in the past
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This may also be true for nicotine biosynthesis, given the known subcellular localisation of NaGR (A622) to the cytosol 32 , and NicGS (BBL) to the vacuole 30 , and also given that nicotine accumulates in vacuoles of tobacco root cells during biosynthesis 38
Yet he likely he did more for the war effort by appearing in films that glamorized the military
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