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While we did not observe any high levels of acute toxicity associated with short-term CSE-EV exposure in this study, we did find that over the course of the 16-week long-term treatment period, CSE-EV-treated SV-HUC cells tended to grow increasingly slowly, ultimately exhibiting near-total growth arrest 4 weeks after the completion of the long-term colony formation assay (data not shown)

The supernatant was used to examine the enzyme activity

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Answer: Addiction to substances like alcohol, cocaine, methamphetamine, opioids, and benzodiazepines is driven by their ability to hijack the brains reward system, creating cycles of cravings and compulsive use