Its also important to read the label and ensure the supplement contains only vitamin B12 and no other additives
(Solna, Sweden: European Centre for Disease Prevention and Control)
If you were working with the 10mg vial, you were dealing with a pre-mixed solution at 2.5mg per 0.5ml
Can give similar symptoms to low B12, you might be deficient in both I'm curious about your overrange folate
On the day of assay, the mannitol and sucrose (MAS) buffer (1) was prepared by diluting sterile-filtered MAS 3 in distilled water, resulting in a solution with the following final concentrations: 220 mM mannitol, 70 mM sucrose, 10 mM KH 2 PO 4 , 5 mM MgCl 2 , 2 mM HEPES, 1 mM EGTA and 0.2% w/v fatty-acid free BSA (3117057001) (pH 7.2), and warmed to 37 C