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Male BALB/c-nu nude mice aged 4 weeks and weighing 18 ~ 22 g were purchased from the Experimental Animal Centre of Southern Medical University (Guangzhou, China) and housed in a specific pathogen-free air-conditioned room (25 2 and 65% 5% humidity) with a 12-h light/dark cycle

It is not intended for human or veterinary use, including cosmetic or topical use

The benzyl alcohol acts as a preservative that inhibits bacterial growth, so a single vial can be accessed for multiple withdrawals across a 28-day period rather than being discarded after one use

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