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The protein was concentrated using Amicon Ultra-4 Centrifugal Filter Units with a 3 kDa membrane cutoff (Merck) and then acidified to a pH of 2.2

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1013 Its antimelanogenic properties result from a variety of mechanisms including stimulation of pheomelanin synthesis rather than darker eumelanin, its antioxidant effects, 14 and interference with intracellular trafficking of melanogenic enzymes

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