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After 10 to 15 years of disease, this pattern progresses in up to 50% of untreated patients, during which time clinical symptoms slowly lead to progressive deterioration over a period of many years, a stage of disease defined as secondary progressive MS (SPMS)

doi: 10.3390/ijms23105354 102 MaoZ

Burgio E, Piscitelli P, Migliore L (2018) Ionizing Radiation and Human Health: Reviewing Models of Exposure and Mechanisms of Cellular Damage

Eng, J., Kleinman, W

RAW 264.7 cells were cultured in complete Dulbeccos modified Eagle medium (DMEM, Gibco) supplemented with 10% (v/v) heat-inactivated fetal bovine serum (FBS) (Avantor Seradigm), 2 mM L-glutamine (Gibco), 1 non-essential amino acids solution (Gibco) and 1 mM sodium pyruvate (Gibco) below 18 passages without exceeding 90% confluency