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The focus is on collagen synthesis, tissue healing, and anti inflammatory balance

In some embodiments, concentration of a dye in any otic formulation described herein is less than 2%, less than 1.5%, less than 1%, less than 0.5%, less than 0.25%, less than 0.1%, or less than 100 ppm of the total weight and/or volume of any formulation or composition described herein

After incubation with the secondary antibody, the cells were washed with PBST and mounted using a Fluoroshield Mounting Medium with DAPI (Abcam ab104139)

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