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The target protein can then be obtained by changing the elution conditions

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All copper peptides share similar potential side effects: temporary redness, irritation with high concentrations, and possible allergic reaction in those with copper sensitivity

Long Journal of the American Chemical Society 1960 82 (4), 795-798 DOI : 10.1021/ja01489a008 This is the paper giving the pK a of water as 15.7 whose methods are criticized in the above articles