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AP/NTS and LPBN sections were incubated at 4 C for 48 h with rabbit anti-Fos antibody (1:500, Cell signalling technology, Switzerland, Cat# 2250, RRID: AB_2247211), and then after PBS washes, incubated with goat anti-rabbit Alexa Fluor CY3 (1:200, LuBio Science GmbH, Switzerland for Jackson Immunoresearch, Cat# 111-165-144) or alexa fluor 488 for 2 h at room temperature

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As the inflammatory response progresses, a glial scar is formed around the injury, consisting mostly of proteoglycans and reactive astrocytes

Most gastrointestinal effects have been observed to diminish with continued exposure and gradual dose titration