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The filter was washed two times with 100 L of UA, centrifuged at 14,000 g for 15 min, and incubated with 100 L of IAA solution (0.05 M iodoacetamide in UA) for 20 min in the dark

Chemical techniques might potentially harm the quality of food, while many physical techniques are costly (Zhi et al

136 Therefore, the immune effects of mTOR inhibitors in prolonging lifespan and healthspan deserve further investigation

They comprise an equal number of males and females aged 39 to 52 years old (average age 44.70 4.94 years)

doi: 10.1016/j.fertnstert.2019.07.007 63 YamagataYNakamuraYUmayaharaKHaradaATakayamaHSuginoNet al