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It significantly stimulates the production of vascular endothelial growth factor, interleukin-6, and TNF- in women with endometriosis

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Expression and purification of recombinant APEX2 APEX2 was expressed from a plasmid (72558 pTRC-APEX2, Addgene) in Escherichia coli BL21(DE3) (EC0114, Thermo Fisher Scientific), as described previously 23

Briefly, mRNA libraries were produced using the Illumina NovaSeq platform where single-end reads were sequenced to a depth of approximately 2025 10 6 reads per sample