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And it's really a question of what you can reasonably do in a study

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LD50 is a crucial number for determining the safety of any drug, and it is typically determined through animal testing, with known LD50 values for common substances like caffeine and aspirin 4m6s

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Notably, animal models have demonstrated that transferring dysbiotic microbiota from CFS/ME patients to germ-free mice can induce fatigue-like behaviors, supporting a causal role for the microbiome in disease pathogenesis ( 3.5.4 Gut microbiota-based therapeutic interventions 3.5.4.1 Probiotics Given these findings, probiotic supplementation, particularly with strains of Lactobacillus and Bifidobacterium , has shown potential to restore microbial balance, enhance SCFA production, and reduce inflammation in CFS/ME patients (Skjevling et al., 2024)