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Group 1: Normal Control (NC) Group 2: High Cholesterol Diet Control (HCD), animals receiving an atherogenic food for 50 days Group 3: Animals receiving high cholesterol diet for 50 days, after this period the animals are fed for eight days by the standard food and receiving by gavage virgin olive oil (1% of body weight) (HCD+VOO)

Among antioxidant enzymes, our recent studies have shown evidence of impaired functioning of the enzyme paraoxonase 1 (PON1) associated to high-density lipoprotein (HDL) in individuals with AD, with reduced protein levels and enzyme activity (60, 61)

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For this, we introduced a second copy of gspD under the control of the copper-inducible promoter, P cuoA at the attB site 75 into our clones that had successfully integrated the deletion plasmid