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The number of living cells were counted by using an automated cell counter (Countess II, Thermo Fisher Scientific Inc.) after staining with trypan blue by pipetting for around 10 s

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[68] Chemistry [edit] Although in chemical terms, GABA is an amino acid (as it has both a primary amine and a carboxylic acid functional group), it is rarely referred to as such in the professional, scientific, or medical community