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Weigh Fmoc-Glu-OAllyl (40.9 g, 100.0 mmol) and HOBT (16.2 g, 110.0 mmol) and dissolve them in 150 mL of DMF, add DIC (20.4 mL, 120.0 mmol) under ice bath for activation for 5 min, and add the mixture to the reaction In the column, the reaction was carried out at room temperature for 2 hours

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Along with the appearance of a considerable number of endothelial lesions following ligation, an initial blood flow deprivation and a presumably inadequate response, decreased NO levels (Figure 7) and increased MDA levels (Figure 8) were observed in the colon tissue