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Peptide Vials (DSIP, 5 mg each): 8 weeks at ~200 mcg/day avg 23 vials 12 weeks at ~200 mcg/day avg 34 vials 12 weeks at ~300 mcg/day avg 56 vials Insulin Syringes (U-100): Per week: 7 syringes (1/day) 8 weeks: 56 syringes 12 weeks: 84 syringes Bacteriostatic Water (10 mL bottles): Use ~3.0 mL per vial for reconstitution

Without the addition of glutathione, the pathway, briefly reconstituted by the addition of methylcobalamin alone will sputter, like an unused engine, creating inefficient function, and an imbalance of related methylation reactions producing an abundance of pathway inhibiting substances like SAH

Materials and Methods Cell lines FlpIn 293 cells (Life Technologies) with low passage number were transfected with empty pcDNA5/FRT vector or pcDNA5/FRT vector containing either wild-type MRP1, wild-type MRP2, or MRP1/MRP2 chimeras and mutants

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Tissue and Cell 79, 101953