26, 27 Primary antibodies included anti-phospho-p65 antibody (Cell signaling #3033, 1:1000), anti-p65 antibody (Cell Signaling #6956, 1:1000), anti-IB antibody (Cell Signaling #3033, 1:1000), anti-IB antibody (Cell Signaling #9242, 1:1000), anti-phospho- IB antibody (Cell Signaling #6956, 1:2859) and anti--tubulin (Sigma-Aldrich #T-9026, 1:5000)
To quantify light and heavy cysteine-containing peptides, the following parameters were included in the Additional Options pane in the DIA-NN software: fixed-mod Carb, 57.021464, C, label
Because of its unusual chemical bonds, glutathione resists being broken down easily
Always record any reactions observed during testing
[20] It can also be injected intravenously for the purpose of treating cyanide poisoning, as the hydroxyl group is displaced by cyanide, creating a non-toxic cyanocobalamin that is excreted in urine