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Cells were washed with cold PBS and total proteins were extracted with RIPA buffer containing Halt inhibitors (Thermofisher, MA)

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For instance, in an IL-1-stimulated chondrocyte OA model and a surgically induced OA rat model, exosomes derived from OA patient FLS were found to reduce chondrocyte viability, GSH/GSSG ratio, GPX4, SLC7A11, and MMP levels

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