The three men talk about the film, developing its characters, the highs and lows of Hollywood, and at one point early on, Pitt makes a joke about boner pants, but the thing I kept coming back to, the thing that sent a shiver of confusion and foreboding down my spine, was this image of Brad Pitt you see on the left there sitting at a card table, smoking cigarettes, in front of an untouched cheeseburger and an enormous glass of milk
After 8 h transfection, cells were incubated with fresh medium for another 48 h, and subsequently screened with puromycin (2 g/mL) for 72 h
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