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This study explored the roles of the two independent ferroptosis axes, ACSL4/GPX4 and FSP1, in oxalate-induced renal tubular epithelial cell injury, aiming to provide novel therapeutic approaches for treating oxalate-induced AKI

The absorbance at 450 nm was recorded using the microplate reader

Submit the samples to an accredited laboratory for Bac-T testing

Public data analysis Data of mutations, copy number variations, and mRNA overexpression of PSPH were collected from the cBioPortal ( data repository using the publicly available TCGA (The Cancer Genome Atlas) Glioblastoma Firehose Legacy dataset

Results TSCI induces local ferroptosis at the injury site To determine whether ferroptosis occurs at the injury site following TSCI, we assessed several key indicators of ferroptosis