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Let's keep this short and sweet because we're going to get back to the other stuff

Use sterile technique, wipe the stopper before each access, avoid touching sterile surfaces, and keep the vial refrigerated when not in use

Elizabeth Rebello of the University of Texas MD Anderson Center

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The column was then washed with buffer A (300 L, 2,000 g , 2 min), and 10 mM aqueous NH 4 HCO 3 containing 5% CH 3 CN (300 L, 2000 g , 2 min), before peptides were eluted from the column as 15 fractions using 300 L buffer containing an increasing concentration of CH 3 CN in 10 mM NH 4 HCO 3 (%CH 3 CN = 7.5, 10, 12.5, 15, 17.5, 20, 22.5, 25, 27.5, 30, 35, 40, 45, 50, 75) (2000 g , 2 min)