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Because of this, if applied to intact cells or tissues, this method requires cell lysis which is usually carried out by acidification with consequent onset of large amounts of GSSG as an artifact

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Genome-wide coverage tracks for combined iCLIP replicates were generated using deepTools 132 , normalized by sequencing depth and total read count, and binned per nucleotide

The day before the experiment, T-47D and resistant T-47D cells were seeded at a density of 1.0 10 4 cells/well in a 6-well plate and incubated for one day at 37C