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BALF was centrifuged to collect supernatants for cytokine and total protein content analysis, while cell pellets were used for differential cell counts via cytospin centrifuge and Hema-3 staining

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Bovine serum albumin, Agarose (low gelling temperature), Prodan, and ADP/ATP ratio assay kit (MAK135) were obtained from Sigma, USA

Most patients notice subtle improvements after 3 to 4 sessions, with more visible results after completing a full course

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