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No lethal dose was identified across a wide dose range from 0.006 mg/kg to 20 mg/kg body weight

The degree of ischemia of the retina was scored 14 by taking images of the animals fundus as follows: 1: orderly eye background, normal presentation of the choroidal blood vessels (normal reddish fundus background color)

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Animal Model Evidence The systemic evidence base is entirely preclinical: Pulmonary fibrosis (mouse model): Intraperitoneal GHK-Cu at 0.2-20 micrograms/g/day protected against bleomycin-induced lung fibrosis by inhibiting TGF-beta1/Smad signaling and reducing oxidative stress (PMID 31809714)