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To address the issue, researchers are actively exploring alternative synthesis methods that minimize or eliminate the need for harsh organic chemicals

A SYBR Premix Ex Taq II (Tli RNaseH Plus) Kit was used to perform qRT-PCR in technical triplicate on a StepOnePlus Real-time PCR System (Applied Biosystems, USA) following the manufacturers instructions

This mechanism has positioned AHK-Cu as a compound of interest for researchers studying skin matrix integrity, structural protein regulation, and age-related changes in connective tissue biology

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M., Gutirrez, J