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Allelic exchange substrates (AES) containing the Kan R flanked by ~1 kb identity arms for site-specific recombination were obtained by high fidelity PCR using BAC Rv412- cobM,K ::Kan R as template and Conf-KO BAC cobMK-Fw/Conf-KO BAC cobMK-Rv primers

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Standard injectable protocols use 0.25-0.5 mg daily via subcutaneous injection, with many researchers noting visible improvements within 5-10 days for minor wounds and 2-4 weeks for more significant skin injuries

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