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Proteins were precipitated again and digested with LysC and trypsin in 200 mM EPPS buffer pH 8.0 at a 100:1 substrate-to-enzyme ratio overnight at 37C

Activation and inhibition of AMPK can affect the differentiation of osteoblasts and osteoclasts, thus acting as a regulatory mechanism of joint bone homeostasis (104, 120)

B., Buckley, C

Following homogenization, 120 L of tissue homogenate was incubated with 0.2 mM mito-TEMPO-H in KrebsHenseleit KHB containing 100 M in 200 L of total volume for 1 h at 37 C