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Protein bands were visualized using Immobilon Western Chemiluminescent HRP Substrate (Merck Millipore, Billerica, MA, USA) and a ChemiDoc MP Imaging System with Image Lab Touch Software Version 1.0 (Bio-Rad Laboratories, Hercules, CA, USA)

The anonymous collection of discarded blood samples from SCD children undergoing chronic transfusion was classified as exempt by the Institutional Review Board at Augusta University and did not require informed consent

TB-500 (Thymosin Beta-4 Derivative) Research Focus: Evaluated for its effects on actin-binding proteins and cytoskeletal organization

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