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Testing of Reducing Effects The stable radical 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay ( Iron Chelating Ability Assay The Prussian blue assay was used for the testing

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Through direct binding to presenilin enhancer 2 (PEN2) on the lysosomal membrane, metformin inhibits v-ATPase via interaction with ATP6AP1, triggering recruitment of the AXIN-LKB1 complex to the lysosome (Ma et al., 2022