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Heat Inactivated: Destroy the enzyme activity by incubating at 80°C for 15 minutes
2nd strand qPCR was performed using Bio-Rad iQ SYBR mastermix and gene-specific primers that yielded 250-350 bp amplicons
* The cells contain an inducible mutant trfA gene whose gene product is required for initiation of replication from the oriV contained on the CopyControl pCC1™ Vectors or on clones that have been retrofitted with CopyControl capability using the EZ-Tn5™<oriV/KAN-2> Insertion Kit
Smaller surface area size
RNA stabilised at room temperature for at least 2 Weeks at Room Temperature
EGTA size:25g Heat Inactivated: Destroy the enzyme
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