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Apply at different times to avoid potential interactions

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Relative number of living cells is defined as the following equation: relative number of living cells = counted living cell number (sample)/counted living cell number (control), where the control is cells cryopreserved by a commercially available cryoprotectant, CultureSure freezing medium (CS-FM, Fujifilm Wako Pure Chemical Corporation)

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This combination occurs when systolic pressure drops below 90 mmHg while heart rate exceeds 100 bpmyour heart speeds up to compensate for reduced blood volume or pumping efficiency