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However, you may want to avoid drinking alcohol at least 24 hours before getting the vaccine to err on the side of caution

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NAFLD appears to be more frequent in industrialized countries, its global prevalence is about 25% and varies across different geographical areas, being higher in Middle East and South America and lower in Africa (33)

One such example involves utilizing a smaller Cas9 ortholog from Staphylococcus aureus (SaCas) (3.15 kb), which is ~1 kb shorter than the SpCas9, so that the CRISPR-Cas9 construct and gRNA can fit within a single AAV vector [159, 160]