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Marcu KB, Otero M, Olivotto E, et al

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In the acute phase, the neuroinflammatory response worsens BBB injury by degrading tight junctions and the extracellular matrix, promoting neuroglial proliferation, and activating cardiomyocytes, thereby further increasing its permeability

However, the RFP-positive/GFP-negative puncta represent the fusion of autophagosome to lysosome to form autolysosome, during which GFP is quenched because of its susceptibility to a low pH in lysosome

AJNR Am J Neuroradiol 41(5):844-851, 2020