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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole

That means MPs affect the fishs behavior, physiology, and metabolism and interfere with the disease resistance in fish populations upon MP accumulation (Mattsson et al., 2015)

A3: Side effects are uncommon but may include mild nausea, stomach discomfort, skin rash, or headache in rare cases

Peptides are short chains of amino acids that signal the body to repair, heal, burn fat, build collagen, or improve hormonal function

In autoimmune diseases, an overactive immune response can damage the body's tissues and deplete its resources, limiting its ability to fight off pathogens and infections