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Characterization of mammalian sedoheptulokinase and mechanism of formation of erythritol in sedoheptulokinase deficiency
HPLC-grade water was produced using a Milli-Q ultrapure water purification system (Bedford, MA, United States)
Estimation of liver function parameters Aspartate aminotransferase (AST), alanine aminotransferase (ALT) were assayed in serum samples obtained from all groups of mice by a colorimetric method of Reitman and Frankel ( Estimation of iron parameters Serum iron (SI) concentration and total iron-binding capacity (TIBC) were estimated by colorimetric analysis according to the manufacturers protocols for commercial kits (Biodiagnostic, Egypt)
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